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Journal: Communications Biology
Article Title: Pathology and viral evolutionary dynamics in a hamster model of persistent SARS-CoV-2 infection
doi: 10.1038/s42003-025-09473-y
Figure Lengend Snippet: a Lung, b ileum, and c liver tissues were collected 100 days after inoculation with the XBB.1.16 variant from hamsters #1, #2, and #4. Representative images of the tissues at high magnification are shown for immunohistochemistry (IHC) with a rabbit monoclonal antibody that detects SAS-CoV-2 nucleocapsid; in situ hybridization (ISH) targeting the nucleocapsid gene of SARS-CoV-2; immunohistochemistry with a rabbit polyclonal antibody that detects the macrophage marker Iba1; and hematoxylin and eosin (H&E) staining. Scale bars, 100 µm.
Article Snippet: Tissue sections were also processed for IHC with a rabbit monoclonal antibody against the viral
Techniques: Variant Assay, Immunohistochemistry, In Situ Hybridization, Marker, Staining
Journal: Communications Biology
Article Title: Pathology and viral evolutionary dynamics in a hamster model of persistent SARS-CoV-2 infection
doi: 10.1038/s42003-025-09473-y
Figure Lengend Snippet: a Number of iSNVs detected over time in oral swabs from each hamster compared to the virus inoculum. b Cumulative distribution of nonsynonymous and synonymous iSNV frequencies detected in oral swab samples shown for each hamster. The y -axis shows the proportion of iSNVs with frequencies that fall below the frequency values given on the x -axis. c The ratio of nonsynonymous (NS) to synonymous (S) iSNVs in each open reading frame compared to neutral expectation (gray bars, calculated from the ratio of potential nonsynonymous to synonymous sites). For the E (envelope) open reading frame in which we detected no synonymous substitutions, we set the NS/S value to 6. Black whiskers show the 95% confidence interval of the ratio. Overall, the entire SARS-CoV-2 genome; N nucleocapsid protein, M membrane protein.
Article Snippet: Tissue sections were also processed for IHC with a rabbit monoclonal antibody against the viral
Techniques: Virus, Membrane